Mouse immunoglobulin E (IgE) elisa kit shocked the world in minutes - Database & Sql Blog Articles

Mouse immunoglobulin E (IgE) elisa kit basic information Immunoglobulin E (IgE) is a class of δ chain-like affinity cell antibodies, is involved in the regulation of allergic rhinitis, allergic asthma and eczema Primary antibody. Since the discovery of IgE by Japanese scholar Ishizaka in 1966, IgE research has made significant progress, and IgE receptors have been found on the surface of mast cells, basophils, eosinophils and macrophages, and various allergies. Disease patients, including allergic asthma patients, have isolated specific IgE for a variety of pollen, dust mites, molds and animal skin. In recent years, many cytokines such as IL-4 and γ-interferon have been involved in the regulation of IgE synthesis. IgE antibodies can initiate both rapid-onset allergic reactions and delayed-onset allergic reactions. Color development and colorimetric color development of the mouse immunoglobulin E (IgE) elisa kit is the last incubation step in an ELISA assay in which the enzyme catalyzes a colorless substrate to produce a colored product. The temperature and time of the reaction are still factors that affect color development. Properly increasing the temperature helps to accelerate color development. The negative holes remain colorless for a certain period of time, while the positive holes are colored for a prolonged period of time. In the quantitative test, the reaction temperature and time after the addition of the substrate should be determined according to the regulations. The ELISA coloration result is best carried out by a microplate reader, which can reduce the experimental error and improve the analysis accuracy of the critical value sample. Try to avoid direct judgment of the results by the naked eye, because the visual differences of different individuals. It should be noted that when the developer is added, the developer is kept out of the flow. Avoid adding more bubbles when adding stop solution, otherwise the probability of false positive results may increase. The colorimetric colorimetric method has two methods: a visual method and an enzyme standard colorimetric method. The visual method is simple and straightforward, but for the same specimen, the operator's difference sometimes has different results and has certain subjectivity. Colorimetric results are usually expressed in terms of optical density and are now expressed as absorbance as specified. The microplate reader should not be placed under sunlight or strong light. The suitable room temperature during operation is between 15 °C and 30 °C. Preheat the instrument for 15~30 minutes before use, so that the measured results are more accurate. Before colorimetry, clean the liquid attached to the bottom of the plate with a clean absorbent paper, and then place the plate correctly on the colorimetric frame of the enzyme-based colorimeter. In summary, high-quality reagents, good instrumentation and correct operation are necessary conditions to ensure accurate and reliable ELISA results. In the actual application process, the operator must be proficient in basic operational skills, conduct strict and careful verification of each link, try to avoid the occurrence of false positive and false negative reactions, ensure the test results are true and reliable, and provide reliable diagnosis for clinical diagnosis and disease prevention. The theoretical basis. Purchase the company's elisa kit, enjoy zero-cost shipping, and improve after-sales. In addition, you can enjoy the authoritative enzyme-linked immunoassay service, skilled operators, and sophisticated laboratory equipment to ensure that each experimental data is true and reliable. The ELISA kits sold by the company are of quality assurance! Technical guidance is provided throughout the sale and after sale, and there are quality problems, which can be returned free of charge!