The voles' sex hormone elisa kit is available from stock. Shanghai Jinma Biotech Co., Ltd. specializes in providing various kinds of ELISA kits for ELISA kits. If you purchase our ELISA kit, you can enjoy free detection service. Recently, Liao, a student from Sichuan University, inquired about our company, saying that the Elisa kit that I ordered was a bit problematic. I hope the technology gives some answers.
In the experiment of voles sex hormone elisa kit, we need to pay attention to some experimental error problems. The following steps are taken by Shanghai Jinma Technology to analyze the error of the experiment:
1. Shake all reagents thoroughly before use. Do not allow the liquid to generate a large amount of foam, so as not to add a large amount of bubbles during the experimental loading, and errors in the loading during the experiment.
2. The number of slats required is determined by the number of samples to be tested plus the number of standards. Multiple holes are recommended for each standard and blank hole. Each sample should be determined according to its own quantity. For example, if you can use the double hole in the experiment, try to make the double hole.
3. 50 ul of the diluted standard was added to the reaction well, and 50 ul of the sample to be tested was added to the reaction well. Immediately add 50 ul of biotinylated antibody. The membrane was covered, gently shaken to homogenize, and incubated at 37 ° C for 1 hour.
4. After the above experiment, the liquid in the well was removed, and each well was filled with the washing liquid. After shaking for 30 seconds, the washing liquid in the well was removed and patted dry with absorbent paper. Repeat the operation 3 times. If washing with a washing machine, the number of washings needs to be increased once.
5. 80 ul of affinity streptavidin-HRP was added to each well, shaken gently by shaking, and incubated at 37 ° C for 30 minutes.
6. The liquid in the well was removed, and each well was filled with the washing liquid. After shaking for 30 seconds, the washing liquid was removed and patted dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once.
7. Add 50 μl of each of the substrates A and B to each well, shake gently by shaking, and incubate for 10 minutes at 37 °C. Note: Avoid lighting.
8. When the ELISA plate was taken out, 50 ul of the stop solution was quickly added, and the result was measured immediately after the addition of the stop solution.
9. Finally, the OD value of each well was measured at a wavelength of 450 nm.
Our company is proficient in international advanced technology and information, and exerts its best in the detection of ELISA test kits. Its reliability, sensitivity, repeatability, and specificity ensure the perfect curve of the experimental results, winning customers' multiple purchases.
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