What is the meaning of the recovery rate of 120% in the elisa kit? - Huaqiang Electronic Network

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

CSTCR5M57G53-120

As we all know, the elisa kit needs to look at the recovery rate when testing. What is the meaning of the recovery rate of 120%? Let's listen to Xiaobian analysis.
The recovery rate is the degree of loss of the analyte in the sample analysis process. The less the loss, the higher the recovery rate. If the standard solution is 1 PPM, it is 1 mg/L, and the standard data is 0.99 mg/L. Your recovery rate is 99%, which is closely related to the real ingredients, indicating the accuracy of the method. For example, the determination of total inorganic chlorine in water, the sample water contains inorganic chlorine 20

Mg

/L, take 100

mL

Water sample to be measured, add 0.1

mL

Concentration is 10

Mg

/

mL

Standard sample containing inorganic chlorine, the volume change is neglected in the measurement, if the inorganic chlorine in the sample is determined to be 2.98

Mg

/L, the recovery rate is 98%, I don't know if this explains why the landlord understands. For quantitative testing, it is mainly a verification of accuracy. However, I saw that the content written by others was better than what I wanted to say, so I posted it. Relative recovery may be higher than

100%

If you just say "degree of loss", it is actually not accurate. It should be said that the closer the recovery rate is

100%

, indicating that the accuracy of this kit is higher.
In the recovery test, the recovery rate should be 80%, 100%, and 120% of the standard solution concentration. When making the sample content, it should be as close as possible to the standard solution concentration, so that the linearity of the standard curve may be avoided.
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